文档介绍：Isolation, Identification and
Degradation Characterization of 1,2-dichlorobenzene
Degrading Strain

Huihui Liu Chunsheng Yang Cheng Ding* Zhaoxia Li Huixing Liang
School of Chemical and Biological Engineering, Yancheng Institute of Technology, Jiangsu
Yancheng 224051 China
E-mail:ycdingc@
Abstract—A bacterium(strain DL-1) capable of growing with1,2- The aim of this work was to develop a method for
dichlorobenzene as sole carbon and energy sources was isolated isolating facultative aerobic 1,2-dichlorobenzene biodegrading
from rhizosphere soil in phragmites wetland by using the anism from the reed rhizosphere soil in Yancheng
enrichment culture contaminated with1,2-dichlorobenzene wetlands, to probe into the purified anism responsible
concentration gradient of 20, 40, 60,80, 120, 160 and 200 mg•L- for 1,2-dichlorobenzene at different concentrations in the
1. It was identified as Bacillus cereus by 16S rDNA gene sequence minimal fluids culture, and to obtain information about the
analysis and the bacterial culture characteristics, carried degradation characterization of the tested strain in terms of the
physiological and biochemical research, the strain was Gram- ability of 1,2-dichlorobenzene biodegradation.
positive, peritrichous flagellum 。 The further degrading
characterization reserch proved the strain was a facultative
anaerobe and t