文档介绍：Listeria monocytogenes Sigma B contributes to the tolerance of bile
stress in the gastrointestinal tract
YINGYING FENG#, QIANG ZHANG#, LI WANG, FEIFEI FENG, QIN LUO*
Hubei Key Laboratory of ic Regulation and Integrative Biology, College of Life Science, Central China Normal
University, Wuhan, China, 430079
the elevated osmolarity, alkalescence and bile salts of the
Abstract—The ability of Listeria monocytogenes resisting bile
upper small intestine. The ability of L. monocytogenes to sense
stress is very important for its essful infection and
colonization in the human gastrointestinal tract. Some studies and respond to these nonspecific intestinal hostile
[2]
have indicated that the alternative sigma factor Sigma B (σB), environments is essential for initiating a essful infection .
encoded by sigB, is at least partly involved in bacteria resisting
many adverse conditions. In order to investigate the role of L. Blie salt is the most ponent of bile, which
monocytogenes Sigma B in bile tolerance in the gastrointestinal facilitates fat digestion and absorption[3]. Bile represents a key
tract, sigB mutant strain EGDΔsigB was constructed, and in challenge to bacteria surviving in the human gastrointestinal
parallel with wild type strain EGD, exposed to 3% bile salt( tract. Disruption of the loci involving in bile metabolism often
sublethal concen