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2010全国生物材料大会论文集之 (151).pdf

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2010全国生物材料大会论文集之 (151).pdf

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2010全国生物材料大会论文集之 (151).pdf

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文档介绍:Identification and Expression of Genes Involved in
Race-specific Blast Resistance in Rice

Hai -Yan Hu
Kang-Le Zheng · Jie-Yun Zhuang
Key Discipline Open Laboratory on
National Center for Rice Improvement and State Key
Crop Molecular Breeding
Laboratory of Rice Biology
Henan Institute of Science and Technology
China National Rice Research Institute
Xinxiang 453003, China
Hangzhou 310006, China
e-mail: haiyanlij@
Rong-Yao Chai Yu-Chuan Li
School of Life Science and Technology
Institute of Plant Protection and Microbiology
Beijing Institute of Technology
Zhejiang Academy of Agricultural Science
Beijing 100081, China
Hangzhou 310021, China


Abstract—In this study, differentially expression genes were are rare, partly due to our limited knowledge of resistance
isolated from a indica rice cultivar Zhong156 with a race-specific mechanisms. Therefore, a need to identify new sources is
blast resistance gene subjected to the avirulent race ZC15 and imperative for the durable resistance breeding, which is the
the virulent race ZB1 of Magnaporthe grisea at the seedling most effective and environmentally friendly strategy for
growth stage. Twenty-five cDNA clones were isolated by using control of the disease.
suppression subtractive bined with reverse
Northern blot and confirmed by RT-PCR. The categorization of O