文档介绍：脂质体介导hTERT反义寡核苷酸对HL60细胞增殖及凋亡的影响
作者:关则兵潘学谊蔡小燕
【摘要】目的研究经脂质体介导转染端粒酶逆转录酶(hTERT)反义寡核苷酸(ASODN)对HL60细胞增殖、凋亡的影响。方法运用四唑盐比色(MTT)法检测HL60细胞的增殖、Annexin VFITC/PI双标记法细胞早期凋亡流式检测法检测HL60细胞的凋亡。结果 hTERT ASODN组(终浓度10 μmol/L)、脂质体介导hTERT ASODN组( μmol/L)均能抑制HL60细胞增殖,诱导HL60细胞凋亡,两组比较差异无显著性。各ASODN组对HL60的抑制作用及诱导凋亡作用均弱于阿糖胞苷(终浓度10 μmol/L)(P<)。结论脂质体的介导增强了hTERT AS0DN抑制HL60细胞增殖及诱导凋亡的作用效果。脂质体介导hTERT ASODN有可能成为化疗外治疗急性白血病的有益补充。
【关键词】脂质体;端粒酶逆转录酶;反义寡核苷酸;HL60细胞;凋亡
Abstract:Objective To investigate the effects of liposomemediated hTERT ASODN on the proliferation and apoptosis of HL60 cells. Methods To detect inhibition rate of HL60 multiplication by MTT and detect apoptosis by Annexin VFITC/PI double label method with FCM. Results The hTERT ASODN(10 μmol/L)and liposome
mediated hTERT ASODN( μmol/L) all had the inhibitory effects on proliferation and inducing apoptosis of HL60 cells, but there was no significant difference between the two groups. The inhibitory effects on cell proliferation and inducing apoptosis in the cytarabine group was stronger than the others(P<). Conclusions The inhibitory effects on cell proliferation and inducing apoptosis of hTERT AS0DN were enhanced by liposome. Liposomemediated hTERT ASODN could be an additional method to treat acute leukemia besides ;telomerase reverse transcriptase;antisense oligodeoxyn
Key words:lipo