文档介绍：
鹅 CD4 与 CD8α实时荧光定量 PCR 检测方
法的建立#
赵秋荣,陈舜
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( 四川农业大学预防兽医研究所, 成都, 611130)
摘要:CD4+ T 又被称称辅助性 T 淋巴细胞,CD8+ T 又称细胞毒性 T 淋巴细胞。在细胞免
疫应答中都发挥很重要重称重。在在在中,根据我们前期获得重四川白鹅 CD4、CD8α重
cDNA 序列,设计并合成特异性引物,建立了一种能够实时检测鹅 CD4、CD8α分子转录水
平重荧光定量 PCR 检测方方。两个基因重标准曲线 R2 大于 ,表明线性度良好,扩增效
率也都高于 90%,证明建立重检测方方是可行重。在此基础上,利重分离重鹅脾脏淋巴细
胞称作体外试验重细胞模型,重我们建立方方来检测鹅 CD4、CD8α分子在体外实验模型中
重生物学活性,研究发现 PHA 可以诱导 CD4+、CD8+T 细胞重活化。在检测方方重建立作
进一步研究鹅 CD4 与 CD8α分子重生物学活性以及免疫学活性重奠定了基础。
关键词:四川白鹅;CD4;CD8α; 荧光定量 PCR
中图分类号:S835
Quantification of Chinese goose CD4 and CD8α mRNA
using real-time reverse transcription polymerase chain
reaction assay
ZHAO Qiurong, CHEN Shun
(Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan,
611130, . China)
Abstract: CD4+ T cells, or helper T cells, and CD8+ T cells, or killer T-cells, execute different
aspects of T cell mediated adaptive immunity. Several specific primers were designed according
to the cDNA sequences of goose CD4 and CD8α, and the goose β–actin was used as internal
control. The correlation coefficient (R2) values of the standard curves for all target genes were
above , and qPCR efficiency for all target genes were all above 90% .In this study, the rapid
and specific real-time qPCR methods to analyze the transcription profiling of goose CD4 and
CD8α gene were established, and the speci