树枝形分子与鸟苷酸分子相互作用的定量分析.doc

文档介绍：
树枝形分子与鸟苷酸分子相互作用的定量
分析#
胡婧婧1,程义云2,徐铜文1**
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(1. 中国科学技术大学化学与材料科学学院功能膜研究室,合肥 230026;
2. 华东师范大学生命科学学院纳米医药与生物材料实验室,上海 200062)
摘要:在本项目中,采用核磁共振技术(NMR)对树枝形分子/鸟苷酸(GMP)之间的结合
/包裹进行了深入的定量探索。一维核磁谱图中 G5 树枝形分子最外层的亚甲基质子向低场发
生了剧烈的移动,说明树枝形分子表面阳离子伯胺基团与 GMP 分子中的阴离子磷酸根之间
形成了离子对结构。此外,通过一维核磁谱图中树枝形分子内部质子峰的位移变化值大小计
算出每一个 G5 树枝形分子平均能结合 107 个 GMP 分子,二者之间的结合常数为 17400 M-1。
关键词:高分子化学与物理;树枝形分子;鸟苷酸;相互作用;定量分析
中图分类号:O631
Quantitative analysis of the interaction between dendrimer
and GMP
HU Jingjing1, CHENG Yiyun2, XU Tongwen1
(1. School of Chemistry and Material Science, University of Science and Technology of China,
HeFei 230026;
2. Lab for Nano-medcine and Biomaterials, School of Life Sciences, East China Normal
University, ShangHai 200062)
Abstract: In this study, the binding/ encapsulation between dendrimers and GMP molecules have
been investigated by NMR technique with a quantitative method. 1H NMR spectra showed a
significant downfield shift of methylene protons in the outmost layer of the G5 dendrimer,
indicating the formation of ion pairs between cationic amine groups of dendrimer and anionic
phosphate groups of GMP. The chemical shift titration results showed that the binding constant
between G5 dendrimer and GMP is 17400 M-1, and each G5 dendrimer has 107 binding sites for
GMP.
Keywords: polymer chemistry and physics; dendrimer; guanosine monop