文档介绍：Induction of cytochrome CYP4F3 in hydroquinone-treated HL-60 cells
yuhong Li1,2,hong Wang1, jing Hu1, zhongling ZHu1, zhiwei ZHao1, yongyi Bi*1
1 School of Public Health, 2 School of Medicine,
Wuhan University, Wuhan University of Science and Technology,
Wuhan, Hubei 430071, China Wuhan, Hubei 430065, China
e-mail:******@wust. *corresponding author
Abstract—Cytochrome P450 4F3 (CYP4F3) development of leukemia through this
catalyzes the inactivation of leukotriene B4 by mechanism[7].
ω-oxidation in human neutrophils. Here, we In the past study, we found CYP4F3 was
examined the mRNA and protein expression of the up-regulated consistently among all female
CYP4F3 in HL-60 cells, an acute promyelocytic shoe-manufacture workers with different
leukemia cell line, in the presence of hematological disorders by microarray analysis.
hydroquinone(HQ). We found hydroquinone led to
a decrease in cell proliferation and a increase in cell The purpose of the present study was to delineate
apoptosis in a dose-dependent and time-dependent and to tentatively elucidate the expression of
manner, it also most strongly induced the expression human CYP4F3 in hydroquinone-treated HL-60
of the CYP4F3. CYP4F3 appears to be responsible cells.
for the cell proliferation and cell apoptosis, which
may be play a role in the toxicity of hydroquinone. II. MATERIALS AND ME